how to calculate ic50 in graphpad prism 8

Kilcoyne M, Patil V, O’Grady C, Bradley C, McMahon S (2019) Differential glycosylation expression in injured rat spinal cord treated with immunosuppressive drug Cyclosporin-A. Key wordsįlannery A, Le Berre M, Pier GB, O’Gara JP, Kilcoyne M (2020) Glycomics microarrays reveal differential in situ presentation of the biofilm polysaccharide poly- N-acetylglucosamine on Acinetobacter baumannii and Staphylococcus aureus cell surfaces. In the example used to illustrate the method in this chapter, IC 50 calculation is demonstrated for inhibition of Maackia amurensis agglutinin (MAA) binding to 3′sialyl- N-acetyllactosamine (3SLN) using free lactose. In this chapter, we describe methods to fluorescently label a lectin, to carry out a lectin binding inhibition experiment on glycan microarrays, and to calculate the IC 50 value of a binding inhibitory molecule using GraphPad Prism software. IC 50 values can be calculated using experimental data from various platforms including enzyme-linked immunosorbent assay- (ELISA-)type microtiter plate assays, isothermal titration calorimetry (ITC), or glycan microarrays.

Half maximal inhibitory concentration (IC 50) is a measurement often used to compare the efficiency of various carbohydrates and their derivatives for inhibition of lectin binding to particular ligands.

How to calculate ic50 in graphpad prism 8